Identification, biology, control and management resources identification notes for spodoptera litura and spodoptera littoralis and some native spodoptera moths usda aphis ppq steps for the dissection of male spodoptera moths and notes on distinguishing s. Inhibition of spodoptera frugiperda phenoloxidase activity. Characterization of an egyptian spodoptera littoralis. For publication in journals, books or magazines, permission should be obtained from the original photographers with a copy to eppo. Recent developments in transcriptomic approaches enable the molecular basis of olfaction to be studied even in species with limited genomic information. Spodoptera litura is also known as the oriental leafworm moth, cluster caterpillar, cotton leafworm, tobacco cutworm, tropical armyworm, taro caterpillar, tobacco budworm, rice cutworm, and cotton cutworm. Spodoptera littoralis 7 records new host record cercospora apii s. Free fulltext pdf articles from hundreds of disciplines, all in one place bacillus thuringiensis cry1c resistance development and its processing pattern in egyptian cotton leaf worm. Field key to late instars of spodoptera of america north of mexico steven passoa the following key, based on levy and habeck 1976, passoa 1991, godfrey 1987, and pogue 2002, will facilitate the identification of ten spodoptera species known to occur in the continental united states and canada. Capparidaceae were evaluated in the laboratory for their antifeeding and insecticidal effect on larvae of the cotton leafworm, spodoptera littoralis boisduval lepidoptera, noctuidae, using a leaf dipping. Abstractplants under herbivore attack emit volatile organic compounds vocs that can serve as. Evaluation of two eco friendly botanical oils on cotton leaf worm. Study of the biological cycle of spodoptera littoralis boisduval at different temperatures.
Matings where one or both adult moths had been treated as larvae showed no significant. Comparative assays of spli npv and ac mnpv against 2nd instar larvae of spodoptera littoralis revealed 4fold greater susceptibility of s. The egg of spodoptera littoralis is spheroidal, measuring 0. It is a publicly available structured database with insect pest sequences which will allow identification of a number of genes and comprehensive cloning of gene families of interest for scientific community. We evaluated the impact of bacterial rhabduscin synthesis on bacterial virulence and phenoloxidase inhibition in a spodoptera model. Eggs are somewhat flattened, spherical, and laid in clusters with a felt. It shows only one micropylar area, with the typical figure of a marguerite localized on the apical zone fig. Beet armyworm, spodoptera exigua hubner, 1808 lepidoptera, noctuidae, and flight dynamics data in the sombor region. Effect of the extracts of the spiderflower, cleome arabica. Varied response of spodoptera littoralis against arabidopsis. Characterization of a baculovirus of spodoptera litura lepidoptera. Quantitative rtpcr for vsp2 gene expression 24 h after feeding by spodoptera littoralis larvae. Spodoptera littoralis, also referred to as the african cotton leafworm or egyptian cotton leafworm or mediterranean brocade, is a species of moth in the family noctuidae.
Mar 24, 2014 in lepidoptera, the behavior of caterpillars to plant odors is poorly known. This pest was first recorded in africa in 2016, but detailed information on its distribution and damage and farmers response in invaded areas are largely lacking. Comparative assays of splinpv and acmnpv against 2 nd instar larvae of spodoptera littoralis revealed 4fold greater susceptibility of s. Spodoptera littoralis survivors to a npv infection, regardless of the instar 3rd, 4th, 5th or 6th and the moment when the virus was fed as newly molted instar or 2436 h old instar, did not show differences in female and male larval and pupal periods and the adult emergence was greater than 90%. Data set for diet specific differential gene expression. However, caterpillars are equipped with a reduced number of olfactory sensilla 3 on the antenna and 45 on the maxillary palps which they can use to make fine discrimination between complex plant odors. Aug 01, 2014 the genus spodoptera comprises 31 species, 4 of which are listed as quarantine pests for the european union. Identification of stimuli that trigger upwind flight attraction toward host plants is a current challenge, and is essential in developing new, sustainable plant protection methods, and for furthering our understanding of plantinsect interactions. Spodoptera littoralisinduced lectin expression in tobacco core. A key to the imagines of the eight species occurring in africa and the near east is presented, together with keys to the larvae and pupae of most of these species, including all those of economic importance in this region, as well as brief notes for identifying the eggs of six species. Contact individual photographers for permission to use for any purpose. Terpenoids are toxic compounds produced by plants as a defense strategy against insect herbivores.
Pdf insecticides application and the egyptian cotton leafworm. Ijms free fulltext origanum vulgare terpenoids induce. The egyptian cotton leafworm, spodoptera littoralis boisd. In lepidoptera, the behavior of caterpillars to plant odors is poorly known. Differential response of male and female spodoptera.
Circadian rhythm of glycoprotein secretion in the vas. An egyptian isolate of spodoptera littoralis nucleopolyhedrovirus spli npv was tested for its potential as biocontrol agent in comparison to autographa californica multiple nucleopolyhedrovirus ac mnpv. Feeding and life history of spodoptera exigua lepidoptera. The study of olfaction is key to understanding the interaction of insects with their environment and provides opportunities to develop novel tactics for control of pest species. Inhibition of spodoptera frugiperda phenoloxidase activity by. We first showed that the rhabduscin cluster of the entomopathogenic bacterium xenorhabdus nematophila was not necessary for virulence in the larvae of spodoptera littoralis and spodoptera frugiperda. The genus spodoptera lepidoptera, noctuidae in africa.
Candidate chemosensory genes in female antennae of the noctuid moth spodoptera littoralis. Keys about fact sheets glossary larval morphology references. In this study, we determined faw distribution, genetic diversity. Spodoptera littoralisis a destructive pest of subtropical and tropical agriculture, and has the potential to be a serious pest of glasshouse crops in northern europe. Effects on adult longevity, fecundity and fertility, as well as longterm effects on progeny were determined through oral exposure of spodoptera.
Insects were gathered in 2008 and the culture was renewed with fresh insects from egypt at least once a year through. Spodoptera littoralis larvae damage many agricultural plants, particularly cotton venturini, 1975. Monsanto products are commercialized in accordance with ets product launch. A history of the changes in nomenclature within the genus spodoptera is outlined. Sperm bundles are released from the testis in the evening and are retained in the vas deferens lumen overnight before being transferred to the seminal vesicles. This page was last edited on 10 december 2014, at 19. Aqueous and organic hexane, chloroform, and methanol extracts of siliquae, stems and leaves, and seeds of cleome arabica l. The genus spodoptera lepidoptera, noctuidae in africa and.
Influence of azadirachtin and methoxyfenozide on life parameters of spodoptera littoralis lepidoptera. Growth disruption, abnormalities and mortality of spodoptera littoralis boisduval lepidoptera. The highly polyphagous larvae are frequently devastating crops of importance such as corn, sorghum, cotton and grass. The roughly 30 species are distributed across six continents. We characterized the dynamics of mortality factors affecting immature developmental stages of the fall armyworm, spodoptera frugiperda j. In international trade, the earlier life stages eggs and larvae are being intercepted at point of inspection most frequently, challenging the possibilities of.
Spodoptera eridania cramer, spodoptera frugiperda smith, spodoptera littoralis boisduval, and spodoptera litura f. Abstract resistance to several classes of insecticides was diagnosed in the cotton leafworm clw. Pineda, samuel, anamabel martinez, joseissac figueroa, marcelaines schneider, pedro del estal, elisa vinuela, benjamin gomez, guy smagghe, and flor budia. Effects of the spodoptera littoralis granulovirus on the development. Adults feed on nectar, and females oviposit on the leaves of crop plants. Bacillus thuringiensis cry1c resistance development and its. The spodobase database provides integrated access to expressed sequence tags est from the lepidopteran insect spodoptera frugiperda. Its common names reference two of the most frequent host plants of the moth.
The wildtype autographa californica nuclear polyhedrosis virus induces apoptosis of spodoptera littoralis cells by nor chejanovsky and eduard gershburg download pdf 615 kb. We tested the effect of origanum vulgare terpenoids on the generalist herbivore spodoptera littoralis and the response of the plant to herbivory. To investigate the roles cpks play in a herbivore responsesignaling. Terpenoids were analyzed by gcfid and gcms and quantitative gene expression qpcr was evaluated on selected plant genes involved in both terpene. Common names cotton leafworm egyptian cottonwarm mediterranean brocade moth noctuelle mkditerranknne afrikanischer baumwollwurm rosquilla negra principal hosts 3, 10 polyphagous. Moth photographers group spodoptera frugiperda 9666. Several types of treatments have been used against this pest, but many of them failed because of the rapid development of genetic resistance in the different insect populations. Transcriptome analysis of gene families involved in. Spodoptera littoralisinduced lectin expression in tobacco by gianni vandenborre, otto miersch, bettina hause, guy smagghe, claus wasternack and els van damme download pdf 614 kb. Monitoring for exotic spodoptera species lepidoptera. Capparidaceae were evaluated in the laboratory for their antifeeding and insecticidal effect on larvae of the cotton leafworm, spodoptera littoralis boisduval lepidoptera, noctuidae, using a leaf dipping bioassay with castor bean, ricinus.
Spodoptera litura, otherwise known as the tobacco cutworm or cotton leafworm, is a nocturnal moth in the family noctuidae. Overview of lepidoptera families purdue university. Sep, 2002 reproductive systems of male moths contain circadian clocks, which time the release of sperm bundles from the testis to the upper vas deferens uvd and their subsequent transfer from the uvd to the seminal vesicles. General information about spodoptera littoralis spodli name language. The fungal infection rate of eggs varies depending upon the conidial. The cotton leafworm, spodoptera littoralis boisduval lepidoptera. Overview of lepidoptera families taxonomic workshop for early detection of important tortricidae and other lepidopteran agricultural and silvicultural pests umass amherst 1517 july 20 todd m. Candidate chemosensory genes in female antennae of the. Files are available under licenses specified on their description page. Noctuidae and some native spodoptera moths introduction the purpose of this handout is to help you distinguish two exotic spodoptera moths of concern to u. Here we use transcriptome and expression profiling analysis to. Electrophysiological and behavioral responses of spodoptera.
In this work, we characterized behavioral responses of spodoptera littoralis larvae. Noctuidae is the most common, serious and devastative pests which attack large scale of. The advantage of using this new technique is discussed. A total of 28 species representing 15 fungal genera were found to be associated with the all different stages of the egyptian cotton leafworm,spodoptera littoralis. Spodoptera littoralis, also known as the african cotton leafworm, egyptian cotton leafworm or the mediterranean brocade, is native to africa and israel and widely found in both africa and the mediterranean europe. Noctuidae, feeds on a wide range of important crops and is one of the most destructive pests in the tropical and subtropical areas of the world. The cotton leaf worm, spodoptera littoralis boisd lepidoptera.
All photos included on this page can only be used for educational purposes. Some authors mistakenly continue to regard the allopatric old world cotton leafworm species s. Eppo standards pm 71241 diagnostic protocol for spodoptera littoralis, spodoptera litura, spodoptera frugiperda, spodoptera eridania. View spodoptera littoralis research papers on academia. Maize farmers in subsaharan africa recently experienced unusual damage in their farms, attributed to the fall armyworm faw spodoptera frugiperda j. This ranking characterizes spodoptera littoralis as quarantine significant for the u. Herbivoreinduced maize leaf volatiles affect attraction and. Targeting the diuretic hormone receptor to control the. Department of bioagricultural sciences and pest management. All structured data from the file and property namespaces is available under the creative commons cc0 license. Transcriptome and expression profiling analysis link patterns. Spodoptera littoralis georgia invasive species task force. Herbivoreinduced maize leaf volatiles affect attraction. In the middle of this micropylar area we observe a circular space19.
However, until now this prediction was not testable. The insect olfactory system discriminates odor signals of different biological relevance, which drive innate behavior. Noctuidae on different host plants article pdf available in annals of the entomological society of america 94jul 2001. Spodoptera littoralis spodliphotos eppo global database. The cotton leafworm, spodoptera littoralis, is a phytophagous pest and current focal species for insect chemical ecology and neuroethology. Occurrence, abundance, and efficiency of some local. Purification and identification of an angiotensin i converting enzyme ace inhibitory peptide from the gastrointestinal hydrolysate of the cotton leafworm, spodoptera littoralis. Bacteria with mutations affecting the rhabduscin synthesis. Histone h3lysine9 h3k9 trimethyltransferase gene su var was cloned and identified in three spodoptera insects, spodoptera frugiperda s. In this work, we characterized behavioral responses of spodoptera littoralis larvae to 11 odorants found in. Development and validation of realtime pcr tests for the. Aug 23, 2014 spodoptera frugiperda noctuidae is a major agricultural pest throughout the american continent. Frontiers flight attraction of spodoptera littoralis.
Matings where one or both adult moths had been treated as larvae showed no. Spodoptera littoralis spodlioverview eppo global database. It is a highly polyphagous organism that is a pest of many cultivated plants and crops. Spodoptera littoralis nucleopolyhedrovirus wikispecies. Fieldevolved resistance to the bt maize expressing the cry1ab protein is reducing it efficacy in brazil omoto et al. Plants fed on by second instar larvae are designated by h. Steps for dissection of male spodoptera moths lepidoptera. Fungi associated with the egyptian cotton leafworm spodoptera.
Llttoralls 331 metabolic studies with the cotton leafworm, spodoptera littoralis, indicated that the dealkylation of stigmasteml occurs similarly to the pathway in m. An egyptian isolate of spodoptera littoralis nucleopolyhedrovirus splinpv was tested for its potential as biocontrol agent in comparison to autographa californica multiple nucleopolyhedrovirus acmnpv. It has been recorded several times in the uk, and even though it has been intercepted at u. Pdf effects of the spodoptera littoralis granulovirus on the. We produced male and female illuminabased transcriptomes from chemosensory and nonchemosensory tissues of s. Nov 22, 2019 beet armyworm, spodoptera exigua hubner, 1808 lepidoptera, noctuidae, and flight dynamics data in the sombor region. Spodoptera littoralis can be distinguished from native species by a combination of valve characters. This moth is found in asia, with some specific problematic pest population reports occurring in cambodia, hong kong, india, the. Influence of azadirachtin and methoxyfenozide on life. Targeting the diuretic hormone receptor to control the cotton. Occurrence, abundance, and efficiency of some local parasitoids attacking spodoptera littoralis lepidoptera. To control the attacks of this insect, several types of insecticides have been used, including synthetic pyrethroids, organophosphates, and. Notes on taxonomy and nomenclature top of page for about 60 years, the egyptian cotton leafworm was known as prodenia litura.
Simplified field key to late instars of spodoptera of. Noctuidae adults to azadirachtin and methoxyfenozide. This article is from frontiers in plant science, volume 4. Purification and identification of an angiotensin i. Noctuidae, is one of the most devastating pests of crops worldwide.
Emmanuelle jacquinjoly 1,2, fabrice legeai 3, nicolas montagne 1,2, christelle monsempes 1,2, mariechristine francois 1,2, julie poulain 4, frederic gavory 4, william b. Egyptian cottonworm spodoptera littoralis integrated pest. Pm 7124 1 spodoptera littoralis, spodoptera litura. Rnaseq assembly, contig sequences, blast2go hits against nr database, hit accessions, go annotations and relative expression levels for spodoptera littoralis excel file. Establishment and analysis of a reference transcriptome for.
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